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SRX15823499: GSM6259891: 1_stat_LB30; Bacillus thuringiensis; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 15.7M spots, 2.4G bases, 929.1Mb downloads

External Id: GSM6259891_r1
Submitted by: GME, Micalis, INRAE
Study: A sporulation-independent way of life for Bacillus thuringiensis in the late stages of an infection
show Abstracthide Abstract
The formation of endospores has always been considered as the unique mode of survival and transmission of sporulating Firmicutes due to the exceptional resistance and persistence of this bacterial cell form. In this study, we investigated the behavior of a bacterial population in the late stages of an infection as well as the characteristics of the Spo- bacterial form in the B. thuringiensis/Galleria mellonella infection model. Using fluorescent reporters coupled to flow cytometry as well as molecular markers, we demonstrated that the Spo- cells compose the majority of the population two weeks post-infection (pi) and that these bacteria present vitality signs. However, a protein synthesis and a growth recovery assay indicated that they are in a metabolically slowed-down state. Interestingly, they were found to be extremely resistant to the cadaver environment which proved deadly for in-vitro grown vegetative cells and, more strikingly, did not support the germination of spores. A transcriptomic analysis of this subpopulation at 7 days pi revealed a signature profile of this state. Analysis of the expression profile of individual genes at the cell level suggests that iron homeostasis is important at all stages of the infection, whereas the oxidative stress response seems of particular importance as the survival time increases. Altogether, these data indicate that non-sporulated bacteria engage in a profound adaptation process that leads to their persistence in the host cadaver. Overall design: RNA-seq data for 7 days post-infection (dpi) Bacillus thuringiensis cells extracted from Galleria mellonella cadavers (3 samples) versus 30°C LB in vitro-grown Bacillus thuringiensis cells in exponential (3 samples) and early stationnary growth-phase (3 samples)
Sample: 1_stat_LB30
SAMN29246296 • SRS13514968 • All experiments • All runs
Library:
Name: GSM6259891
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Trizol RNA-Extraction + DNAse treatment ribosomal RNA depletion (RiboZero) + Truseq Stranded Total RNA prep
Runs: 1 run, 15.7M spots, 2.4G bases, 929.1Mb
Run# of Spots# of BasesSizePublished
SRR1977915915,727,9462.4G929.1Mb2023-04-01

ID:
22481835

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